geneOmbio Technologies offers wide range of genomics services. This includes analysis of various types of organisms for gene sequencing, gene expression analysis, mutagenesis and DNA cloning. We use Sanger sequencing for customized gene sequencing projects. Absolute and relative quantitation, gene expression analysis is done on StepOne and StepOnePlus real time PCR platforms. geneOmbio has team of experiences researchers that completed successful cloning projects in prokaryotic and eukaryotic system. We extend our support in cloning service till the protein expression stage and thereafter customers can further proceed with large scale manufacturing using the developed clones.
Site Directed Mutagenesis
Our expert team in Site directed mutagenesis service has generated 100% success rate in generation of various type of site directed mutagenesis studies. Site-directed mutagenesis is very useful for modifying a DNA construct, such as alteration of a restriction site in a vector, insertion or deletion of vector elements, tag addition, tag removal and promoter insertion and exchange.
Real Time PCR
geneOmbio provides real time PCR based absolute and relative quantitation services to various clients. We offer fast assay optimization and short turnaround time for such services. Our all round technical support first identifies the type of experiment required for analysis by discussion with client and then provide all the necessary details to clients for such projects.
We are offering the sequencing services for the specific genes amplified from the plants, animal samples and pathogenic microbes using the Sanger sequencing. The sequencing will help to identify, diagnose and potentially develop treatments for genetic diseases that frequently appear in living beings. This services has received immense appreciation and customer satisfaction.
We provide primer walking services for analysis of the base sequences of PCR products or plasmid, which is not analyzable by single primer extension in one time. Internal primers are designed and prepared with results obtained after performing end sequencing using the primers given or designated by customers. Primer walking report includes assembly and other details.
geneOmbio’s cloning services are customized to meet your specific needs. Your gene of interest can be obtained by PCR, RT-PCR or restriction digestion and cloned into any vector of your choice. Cloning and expression in bacterial as well as yeast system has been our expertise. We provide TA, TOPO cloning and gene expression related cloning in pET and pGEX-T systems.
The SNaPshot is a primer extension-based method that enables multiplexing up to 10 SNPs. We use this system to screen and confirm SNPs, assess DNA methylation, characterize and assemble clones into contigs. SNaPshot method has can be used for any project that include high throughput SNP analysis from same sample. We have successfully established multiplexing in earlier projects.
DNA, the genetic material present in all living organisms is unique to each individual like fingerprints. To evaluate the genetic makeup of an organism, the DNA should be mapped using a technique known as DNA fingerprinting. We are offering the DNA fingerprinting studies for Plants, microbes and animals using RFLP, RAPD, ISSR and SSRs.
All the components necessary to develop the most appropriate assay for your research or discovery target of interest are available from our custom solutions. Additionally, our dedicated project team will guide your project through every step of the process, and constantly keep you informed of our progress. Let us take the hassle out of your hands with a custom solution for you.
Metagenomics is the study of microbial communities sampled directly from their natural environment. This enables analysis of populations including many uncultivable and often unknown microbes. Currently, Metagenomics studies are facilitated by the rapid development of NGS techniques, which is faster and cost effective method of metagenomics studies.
geneOmbio offers Next generation sequencing services on Illumina MiSeq. With this we can offer focused applications such as targeted resequencing, metagenomics, small genome sequencing, targeted gene expression profiling, and more. MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read lengths.